Resolvability of fluorescence lifetime distributions using phase fluorometry
نویسندگان
چکیده
منابع مشابه
Resolvability of fluorescence lifetime distributions using phase fluorometry.
The analysis of the fluorescence decay using discrete exponential components assumes that a small number of species is present. In the absence of a definite kinetic model or when a large number of species is present, the exponential analysis underestimates the uncertainty of the recovered lifetime values. A different approach to determine the lifetime of a population of molecules is the use of ...
متن کاملElimination of fluorescence and scattering backgrounds in luminescence lifetime measurements using gated-phase fluorometry.
A new gated form of phase fluorometry for measuring lifetimes is presented. The technique uses a square-wave excitation and gates the detector on only during the off period of the excitation. Using a long-lived sample, this eliminates or reduces errors from scattered light and short-lived fluorescences. Using a square-wave modulated excitation source with a 50% duty cycle, traditional data trea...
متن کاملEffect of cholesterol on membrane microheterogeneity: a study using 1,6-diphenyl-1,3,5-hexatriene fluorescence lifetime distributions.
The effect of cholesterol on microheterogeneity of liposomes obtained from saturated and unsaturated phospholipids was studied by measuring the fluorescence decay of 1,6-diphenyl-1,3,5-hexatriene (DPH). Data obtained by frequency domain fluorometry have been analyzed either by discrete exponential or continuous lifetime distribution approaches. In egg phosphatidylcholine liposomes, the addition...
متن کاملEstimating Lifetime Earnings Distributions Using Copulas∗
In this paper we illustrate both the usefulness of copulas as a statistical technique for modelling dependence in earnings across the lifecycle, as well as contrast it with more traditional approaches for modelling earnings dynamics that appear in the literature. Our model can be estimated using a two-period panel on earnings, in contrast to more traditional approaches which generally require a...
متن کاملFluorescence lifetime based pH sensing using Resorufin
Accurate, non-contact pH sensing is of particular importance in the biological and clinical sciences. Fluorescence lifetime based pH sensing is potentially more useful than intensity based methods because of the reduced sensitivity to excitation source intensity variations, scattering effects, and photobleaching. In this work, we investigate the variation of fluorescence lifetime with pH for re...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1987
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(87)83383-0